ABSTRACT
Leptin is an adipokine made by fat cells and plays a key role in proliferation, cell survival, migration and immune response. Several studies have suggested that individuals with high serum leptin concentrations would increase the risk of breast cancer. G -2548A polymorphism in the leptin gene is located in the promoter region and is associated with the change of leptin serum level. In this study, the association between G -2548A polymorphism in leptin gene and breast cancer susceptibility was investigated. This case-control study was done on 374 Iranian women. This study was performed from March 2013 to February 2013. Blood samples from 203 women with breast cancer and 171 age [ +/- 5]- matched healthy women were collected. Breast cancer patients were selected from Namazi Hospital in Shiraz city. Genomic DNA was extracted from blood samples. The G -2548A polymorphism of leptin gene was determined using polymerase chain reaction-restriction fragment length polymorphism [PCR-RFLP] method. Data analysis was performed by SPSS version 18. Logistic regression analysis was used for association of breast cancer susceptibility and G -2548A polymorphism of leptin gene. The A allele frequency was 60% in control group and 72% in breast cancer patients. There was a significant association between A allele in -2548 position of leptin gene and breast cancer susceptibility [OR: 1.8, 95% CI: 1.3-2.4, P<0.001]. In the recessive effect of the A allele [comparison between AA vs. AG+GG], AA genotype in - 2548 region of leptin promoter sequence was significantly increased the risk of breast cancer [OR=2.2, 95% CI: 1.5-3.4, P<0.001]. It is concluded that A allele in the -2548 promoter region of leptin gene may act as a recessive allele and increase the breast cancer risk
ABSTRACT
To evaluate the effect of a rehabilitation program on quality of life in breast cancer patients. Fifty-seven patients who underwent modified radical mastectomy, had finished chemotherapy and radiotherapy and received hormone therapy were selected and randomly assighned into case and control groups. Control group was under medical care and the case group in addition to medical care received a rehabilitation program [physiotherapy, education and individual counseling] for two months. Quality of life was measured by the EORTC QLQ C30 before one week and 3 months after rehabilitation in both groups. Before intervention there were statistically significant differences in physical [P< 0.001] and emotional functioning [P= 0.02] in cases compared to control group, while no other significant differences were observed in other scale. One week after the program, differences among two groups in 6 scales were significant indicating a better condition in intervention group. Three months after the intervention the cases showed a statistical meaningful improvments in 11 scales. Rehabilitation programs might improve the quality of life after mastectomy in breast cancer patients